Restriction enzyme digest of DNA

with commercially available restriction enzymes

Digest one or more DNA sequences with commercially available restriction enzymes, and list the cut positions for each of them. Click an enzyme name to load its vendors.

Parameters

Fill in the data to analyse, then run the computation.

Sequence :

Show code

Minimum recognition size for each restriction enzyme

Type of restriction enzyme

Only use this endonuclease

Only restriction enzymes with known bases (no N, R, Y ...)

Include Type IIb restriction enzymes (Two cleaves per recognition sequence)

Include Type IIs restriction enzymes (Non-palindromic and cleavage outside of the recognition site)

When two or more input sequences are searched :

Show only endonucleases showing different restriction patterns for searched sequences.