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BioTools

Chaos Game Representation of DNA FCGR Graphical representation of genomes and oligonucleotide frequencies. Chaos Game Representation of DNA CGR Graphical representation of genomes and oligonucleotide frequencies. Distance among sequences Stats Oligonucleotide frequency based comparison of composition. DNA to protein translation DNA Translate a DNA sequence into its protein sequence. Palindromic sequences finder DNA Locate palindromic motifs in a DNA sequence. GC Content Finder DNA Compute the GC content of an uploaded FASTA file. Melting Temperature (Tm) Calculation DNA Estimate the melting temperature of a DNA duplex. Microarray Data Analysis Stats Adaptive quantification method. Microsatellite repeats finder DNA Detect short tandem repeats in a sequence. Frequency of nucleotides Stats Nucleotide and oligonucleotide frequencies in a sequence. PCR amplification DNA In silico PCR amplification. Protein sequence properties Protein Protein sequence information and derived properties. Protein to DNA reverse translation Protein Reverse-translate a protein into a DNA sequence. Random sequences Tools Generate random DNA, RNA or protein sequences. Reduced alphabets for proteins Protein Redundant or simplified alphabets for proteins. Restriction enzyme digest of DNA DNA With commercially available restriction enzymes. Sequence alignment Aligner Alignment of two DNA, RNA or protein sequences. DNA sequence manipulation DNA Manipulate a DNA sequence and read its properties. Skews generator Stats GC-, AT-, KETO- and oligo-skews from custom sequences. Formula functions Tools Useful formulas for sequence and protein calculations.

BioTools In silico PCR amplification

In silico PCR amplification

A,T,G,C and N are allowed; A+T+G+C must be 10 or more.

One mismatch is allowed in any position of primers.

1

Parameters

Fill in the data to analyse, then run the computation.

Sequence :

Primer 1 (5' -> 3') :

Primer 1 (5' -> 3') :

Allow one mismatch

Maximum length of bands (nucleotides) :